Restriction Enzyme Digest & Electrophoresis

This lab experience involves performing restriction enzyme digests on Lambda DNA or a plasmid of your choice. Students will run their digests on an agarose gel while estimating the size of their expected bands.

Skills Involved

• pipetting accuracy
• gel electrophoresis
• analyzing data
  (instructions for using students’ data to develop and use a standard curve and using Excel to organize and graph data are available)